pFUPI AoT5Cyp

LOCUS       pFUPI AoT5Cyp               11832 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Expresses Aotus TRIM5 CypA fusion.
ACCESSION   .
VERSION     .
KEYWORDS    pFUPI AoT5Cyp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, 
            Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J
  TITLE     Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins 
            engineered from human components.
  JOURNAL   J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub
            2009 Sep 8.
  PUBMED    19741300
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11832
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2611..3821
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3658..3677
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3860..4459
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3860..3879)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4356..4376
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4468..5041
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4635..4652)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4862..4881
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6524..7112
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6577..6597)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6995..7006)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7115..7131)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7116..7132)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7637..7817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7843..7860)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7849..8073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8119..8547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8206..8225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8416..8437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8556..8576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8561..8890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8741..8876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8803..8822
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8938..8985
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9004..9378
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9508..9629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9545..9564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9599..9618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9678..9694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9678..9694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9691..9713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9702..9718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9726..9756)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9771..9792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9909..9926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10080..10668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10160..10179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10839..11699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11462..11481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11700..11804)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
     3841 gactctagct agagccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3901 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3961 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4021 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4081 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4141 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4201 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4261 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4321 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4381 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4441 ccgcaagccc ggtgcctgag aattccgccc ctctccctcc ccccccccta acgttactgg
     4501 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4561 gccgtctttt ggcaatgtga gggcccggaa acctggtcct gtcttcttga cgagcattcc
     4621 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4681 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4741 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     4801 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     4861 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     4921 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4981 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     5041 accggtggtc tagaatggct tccagaatcc tggtcaatat aaaggaggag gtgacctgcc
     5101 ccatctgcct ggaactcctg acagaacccc tgagcctgga ctgtggccat agcttctgcc
     5161 aagcatgcat cactgcaaat cacaaaaagt ctatgccaca ccaaggagag agaagctgcc
     5221 ctttgtgccg gatcagttac tcgtctgaga acctgcggcc taatcggcat ttggtcaaca
     5281 tagtggagag gctcagggag gtcatgctga gcccagagga ggggcagaag gttgatcact
     5341 gtgcacacca tggagagaaa cttgtactct tctgtcagca ggatggaaat gtcatttgct
     5401 ggctttgtga gcggtctcaa gaacaccgtg ggcaccagac attccttgtg gaggaggttg
     5461 cacagaaata ccgagaaaag ctccaggtag ctctggagat gatgaggcag aagcagaagg
     5521 atgctgaaaa gttggaagct gacgtcagag aagagcaagc ttcctggaag attcaaatac
     5581 aaaatgacaa aaccaacatc atggcagagt ttaaaaaacg gagagacatc ctggactgtg
     5641 aggagagcaa agagttgcaa aacctggaga aggaggagaa aaacattctg aaaagacttg
     5701 tacagtctga aaatgacatg gtgctgcaga cccagtccgt gagagtgctc atctcagatc
     5761 tggagcatcg cctgcagggg tcagtgatgg agctgttaca gggtgtggat ggtgtcataa
     5821 aaaggattga gaaagtgact ttgcagaatc caaaaacctt tcttaatgaa aaaaggagaa
     5881 tatttcaaac tcctgatctg aaaggaacac tacaagtgtt taaagagccg acagaagtcc
     5941 aacgctactg ggacgccgcc gcctgggacc ttgtagcatc agccatggtc aatcctactg
     6001 tgttcttcga cattgccgtc gatggcgagc ccttgggccg cgtctccttc gagctgtttg
     6061 cagacaaggt tccaaagaca gcagaaaact ttcgtgctct gagcactgga gagaaaggat
     6121 ttggttataa gggttcctgc tttcacagaa ttattccagg gtttatgtgt cagggtggtg
     6181 acttcacacg ccataatggc actggtggca agtccatcta cggggagaaa tttgatgatg
     6241 agaacttcat cctaaagcat acaggtcccg gtatcttgtc catggcaaat gctggaccca
     6301 acacaaacgg ttcccagttt ttcatctgca ctgccaagac tgagtggttg gatggcaagc
     6361 atgtggtctt tggcaaggtg aaagaaggca tgaatgttgt ggaggccatg gagcgctttg
     6421 ggtgcaggta tggcaagacc agcaaaaaga tcaccattgc tgactgtgga caactttaag
     6481 gatccgttaa cggcgcgccg aattcgatat caagcttatc gataatcaac ctctggatta
     6541 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6601 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6661 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6721 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6781 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6841 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6901 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6961 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7021 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7081 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
     7141 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     7201 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     7261 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7321 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7381 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     7441 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     7501 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7561 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     7621 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7681 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7741 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7801 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     7861 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7921 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7981 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     8041 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     8101 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8161 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8221 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8281 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8341 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8401 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8461 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8521 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     8581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8821 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8881 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     8941 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     9001 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     9061 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     9121 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     9181 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     9241 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     9301 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     9361 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     9421 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     9481 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     9541 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9601 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     9661 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11821 gtgccacctg ac
//